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Please use this identifier to cite or link to this item: http://hdl.handle.net/2237/25198

Title: Efficient In Planta Detection and Dissection of De Novo Mutation Events in the Arabidopsis thaliana Disease Resistance Gene UNI
Authors: Ogawa, Tomohiko
Mori, Akiko
Igari, Kadunari
Morita Terao, Miyo
Tasaka, Masao
Uchida, Naoyuki
Keywords: Arabidopsis thaliana
DNA damage sensor pathway
R gene
Mutation
Salicylic acid pathway
UNI
Issue Date: 25-Mar-2016
Publisher: Oxford University Press
Citation: Plant and Cell Physiology. v.57, n.6, 2016, p.1123-1132
Abstract: Plants possess disease resistance (R) proteins encoded by R genes, and each R protein recognizes a specific pathogen factor(s) for immunity. Interestingly, a remarkably high degree of polymorphisms in R genes, which are traces of past mutation events during evolution, suggest the rapid diversification of R genes. However, little is known about molecular aspects that facilitate the rapid change of R genes because of the lack of tools that enable us to monitor de novo R gene mutations efficiently in an experimentally feasible time scale, especially in living plants. Here we introduce a model assay system that enables efficient in planta detection of de novo mutation events in the Arabidopsis thaliana R gene UNI in one generation. The uni-1D mutant harbors a gain-of-function allele of the UNI gene. uni-1D heterozygous individuals originally exhibit dwarfism with abnormally short stems. However, interestingly, morphologically normal stems sometimes emerge spontaneously from the uni-1D plants, and the morphologically reverted tissues carry additional de novo mutations in the UNI gene. Strikingly, under an extreme condition, almost half of the examined population shows the reversion phenomenon. By taking advantage of this phenomenon, we demonstrate that the reversion frequency is remarkably sensitive to a variety of fluctuations in DNA stability, underlying a mutable tendency of the UNI gene. We also reveal that activities of the salicylic acid pathway and DNA damage sensor pathway are involved in the reversion phenomenon. Thus, we provide an experimentally feasible model tool to explore factors and conditions that significantly affect the R gene mutation phenomenon.
URI: http://doi.org/10.1093/pcp/pcw060
http://hdl.handle.net/2237/25198
ISSN: 0032-0781
Rights: This is a pre-copyedited, author-produced version of an article accepted for publication in [Plant & Cell Physiology] following peer review. The version of record [Plant and Cell Physiology. v.57, n.6, 2016, p.1123-1132] is available online at: http://doi.org/10.1093/pcp/pcw060.
Appears in Collections:1.学術雑誌(トランス)

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